Journal: Molecular Medicine

Article Title: CD5L insufficiency exacerbates skeletal joint damage in rheumatoid arthritis

doi: 10.1186/s10020-026-01456-x

Figure Lengend Snippet: Immune profile of WT and CD5L⁻ mice during CIA. A Serum CD5L levels measured in WT mice after immunization. p -values were obtained using the Kruskal–Wallis test with Dunn’s multiple comparisons. B Frequencies of blood leukocyte subsets in WT ( n = 10) and CD5L⁻ ( n = 10) mice. Leukocytes were phenotyped by flow cytometry: CD11b + cells, monocytes (Siglec-F⁻Ly6G⁻F4/80⁺CD11b⁺); inflammatory monocytes (Siglec-F⁻Ly6G⁻F4/80⁺CD11b⁺Ly6C⁺); and Ly6C⁻ monocytes (Siglec-F⁻Ly6G⁻F4/80⁺CD11b⁺Ly6C⁻). Shaded area indicates mean and SEM of total leukocyte frequency. p -values were calculated using Šídák’s multiple-comparisons test. C Serum cytokine levels in WT ( n = 6) and CD5L⁻ ( n = 6) mice. p -values were calculated using the Mann–Whitney test. D Serum RANKL and cross-linked C-telopeptide of type I collagen (CTX-I) levels on day 56 after immunization. p -values were obtained using an unpaired t -test with Welch’s correction. E Splenic gene expression in non-immunized WT ( n = 5) and CD5L⁻ ( n = 5) mice, measured by RT-qPCR and normalized to Actb (β-actin). p -values were calculated using a two-tailed unpaired t -test with Welch’s correction

Article Snippet: C-telopeptide cross-link type I collagen (CTX-I) was measured by an ELISA (IDS Serum CrossLaps, Immunodiagnostic Systems, Boldon, UK) in undiluted samples; detection limit, 0.11 ng/ml.

Techniques: Flow Cytometry, MANN-WHITNEY, Gene Expression, Quantitative RT-PCR, Two Tailed Test